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  • دکتری (1379)

    انگل شناسی

    دانشگاه تربیت مدرس،

  • کارشناسی‌ارشد (1373)

    انگل شناسی

    دانشگاه تربیت مدرس،

  • واکسن های نسل جدید (DNA vaccine)
  • روشهای جدید درمان بیماریهای انگلی و استفاده از نانو پارتیکل ها و بررسی بیان ژن های درگیر در پیشگیری و درمان بیماری های انگلی

    داده ای یافت نشد

    Immunogenicity of in-silico designed multi-epitope DNA vaccine encoding SAG1, SAG3 and SAG5 of Toxoplasma gondii adjuvanted with CpG-ODN against acute toxoplasmosis in BALB/c mice

    Masoumeh Khodadadi, Fatemeh Ghaffarifar, Abdolhosein Dalimi, Ehsan Ahmadpour
    Journal PapersActa Tropica , Volume 216 , 2021 April 1, {Pages 105836 }

    Abstract

    The causative agent of toxoplasmosis, Toxoplasma gondii (T. gondii), is able to influence the health of humans and other vertebrates. Toxoplasma may cause severe illness in the fetus and immunocompromised individuals. The high incidence and intense damages of Toxoplasma infection clearly shows the need to achieve the safe and suitable vaccine. In this study, an immunoinformatics approach was employed to design a multi-epitope DNA vaccine encoding the T. gondii SAG1, SAG3 and SAG5. The bioinformatic outputs supported the immunogenic and non-allergic natures of multi-epitope vaccine. Thereafter, the protective efficacy of the vaccine was evaluated with/without CpG-ODN adjuvant in a laboratory animal model. BALB/c mice were immunized subcutane

    Immunoinformatic analysis of immunogenic B-and T-cell epitopes of MIC4 protein to designing a vaccine candidate against Toxoplasma gondii through an in-silico approach

    Ali Dalir Ghaffari, Abdolhossein Dalimi, Fatemeh Ghaffarifar, Majid Pirestani, Hamidreza Majidiani
    Journal PapersClinical and experimental vaccine research , Volume 10 , Issue 1, 2021 January , {Pages 59 }

    Abstract

    PurposeToxoplasmosis, transmitted by Toxoplasma gondii, is a worldwide parasitic disease that affects approximately one-third of the world's inhabitants. Today, there are no appropriate drugs to deter tissue cysts from developing in infected hosts. So, developing an effective vaccine would be valuable to avoid from toxoplasmosis. Considering the role of microneme antigens such as microneme protein 4 (MIC4) in T. gondii pathogenesis, it can be used as potential candidates for vaccine against T. gondii.Materials and MethodsIn this study several bioinformatics methods were used to assess the different aspects of MIC4 protein such as secondary and tertiary structure, physicochemical characteristics, the transmembrane domains, subcellular locali

    Multi-epitope vaccine expressed in Leishmania tarentolae confers protective immunity to Toxoplasma gondii in BALB/c mice

    Hamidreza Majidiani, Abdolhossein Dalimi, Fatemeh Ghaffarifar, Majid Pirestani
    Journal PapersMicrobial Pathogenesis , 2021 April 29, {Pages 104925 }

    Abstract

    Current study deals with a novel multi-epitope vaccine designed in silico and its confirmation experiments for potential efficacy in BALB/c mice. Major histocompatibility complex (MHC)-binding and B-cell binding epitopes of five Toxoplasma antigens (SAG1, ROP16, GRA12, MIC4 and M2AP) were predicted. Selected epitopes were fused together using SAPGTP linker, and antigenicity, allergenicity, physico-chemical features, secondary and tertiary structures and validations were all performed via bioinformatics servers. Then, vaccine construct was cloned into pLEXSY-neo 2.1 vector. After Leishmania tarentolae transfection, live recombinant and wild parasites were subcutaneously injected into 6–8 week female BALB/c mice and immune responses were me

    Silybum marianum ethanolic extract: in vitro effects on protoscolices of Echinococcus granulosus G1 strain with emphasis on other Iranian medicinal plants

    A Taghipour, F Ghaffarifar, J Horton, A Dalimi, Z Sharifi
    Journal Papers , , {Pages }

    Abstract

    Therapeutic and Preventive Effects of Morphine Against Leishmania Major and Evaluation the Expression of TLRs and Cytokines in Infected Macrophages in Vitro and in BALB/c Mice

    P Ebrahimisadr, F Ghaffarifar, J Jabari, J Horton, Z Sharifi, A Dalimi, ...
    Journal Papers , , {Pages }

    Abstract

    Synergistic Anti-Leishmanial Activities of Morphine and Imiquimod on Leishmania infantum (MCAN/ES/98/LIM-877)

    F Ghaffarifar, M Foroutan, S Molaei, E Moradi-Asl
    Journal Papers , , {Pages }

    Abstract

    Efficacy of green synthesized silver nanoparticles via ginger rhizome extract against Leishmania major in vitro

    M Mohammadi, L Zaki, A KarimiPourSaryazdi, P Tavakoli, A Tavajjohi, ...
    Journal Papers , , {Pages }

    Abstract

    Toxoplasma gondii in Slaughtered Sheep in High-and Low-Humidity Regions in the South of Iran: Molecular Prevalence and Genotype Identification

    SZ Khademi, F Ghaffarifar, A Dalimi, MS Dayer, A Abdoli
    Journal Papers , , {Pages }

    Abstract

    Synthesis of Tellurium Oxide (TeO2) Nanorods and Nanoflakes and Evaluation of Its Efficacy Against Leishmania major In Vitro and In Vivo

    P Tavakoli, F Ghaffarifar, H Delavari, A KarimiPourSaryazdi, MS Dayer, ...
    Journal Papers , , {Pages }

    Abstract

    Evaluation of Anti-amoebic Activity of Spring Leaf Extract of Artemisia Aucheri on Acanthamoeba (T4 strain) In vitro

    A KarimiPourSaryazdi, Y KarimiPourSaryazdi, F Ghaffarifar, M Ahmadi, ...
    Journal Papers , , {Pages }

    Abstract

    In Vitro and In Vivo Anti-parasitic Activity of Artemisinin Combined With Glucantime and Shark Cartilage Extract on Iranian Strain of Leishmania major (MRHO/IR/75/ER)

    F Ghaffarifar, S Molaei, Z Mohammad Hassan, MS Dayer, A Dalimi, ...
    Journal Papers , , {Pages }

    Abstract

    In Vitro and In Vivo Anti-parasitic Activity of Artemisinin Combined With Glucantime and Shark Cartilage Extract on Iranian Strain of Leishmania major (MRHO/IR/75/ER)

    F Ghaffarifar, S Molaei, ZM Hassan, MS Dayer, A Dalimi, V Nasiri, ...
    Journal Papers , , {Pages }

    Abstract

    Evaluation of the effects of Rumex obtusifolius seed and leaf extracts against Acanthamoeba: An in vitro study

    T Nayeri, F Bineshian, F Khoshzaban, AD Asl, F Ghaffarifar
    Journal Papers , , {Pages }

    Abstract

    Comparison of the Effects of Sambucus ebulus Leaf and Fruit Extracts on Leishmania major In Vitro

    S Kadkhodamasoum, F Bineshian, A KarimiPour, P Tavakoli, M Foroutan, ...
    Journal Papers , , {Pages }

    Abstract

    A study on the in vitro and in vivo effects of aqueous and alcoholic extracts of Salvia mirzayanii on Leishmania major.

    F Hasanzadeh, F Ghaffarifar, S Mehdizadeh, MS Dayer
    Journal Papers , , {Pages }

    Abstract

    Enhancing immune responses by a novel multi-epitope ROP8 DNA vaccine plus interleukin-12 plasmid as a genetic adjuvant against acute Toxoplasma gondii infection in BALB/c mice

    Masoud Foroutan, Mohammad Barati, Fatemeh Ghaffarifar
    Journal PapersMicrobial Pathogenesis , Volume 147 , 2020 October 1, {Pages 104435 }

    Abstract

    BackgroundToxoplasmosis is a widespread zoonotic infection, caused by an obligate intracellular protozoan. The infection is often asymptomatic in immunocompetent individuals, although in persons with impaired immune system may lead to severe and progressive complications. Constant attempts of scientists have made valuable findings in the development of Toxoplasma gondii (T. gondii) candidate vaccines. However, an effective vaccine has not been successfully developed yet. In the current study, we tested the co-delivery of a novel multi-epitope pcROP8 DNA vaccine with a plasmid encoding IL-12 (pcIL-12) to assess the immune responses in BALB/c mice against acute T. gondii infection.MethodsBALB/c mice were immunized on days 0, 21, and 42. The i

    In Vitro Effect of Some Medicinal Plants on Leishmania major Strain MRHO/IR/75/ER

    Mojtaba Raeisi, Kamal Mirkarimi, Behrooz Jannat, Bahman Rahimi Esboei, Abdol Sattar Pagheh, Zahra Mehrbakhsh, Fatemeh Ghaffarifar, Oghlniaz Jorjani, Masoud Foroutan
    Journal PapersMedical Laboratory Journal , Volume 14 , Issue 4, 2020 July 10, {Pages 46-52 }

    Abstract

    Methods: The essential oils were prepared from freshly dried and powdered plants with steam-distilled water. Iranian strain of Leishmania promastigotes was cultured in RPMI medium and the inhibitory effects of different concentrations (25, 32, 62.5, 125, 250, 500 and 1000 μg/ml) of the essential oils were investigated at 24, 48 and 72 hours. The number of live parasites before and after treatment with the essential oils was counted by trypan blue 10% staining and using neobar lam.Results: The essential oils significantly decreased the number of promastigotes in a dose-dependent manner (P< 0.05). However, the inhibitory effects of F. vulgare and R. officinalis essential oils were more profound compared to other essential oils. Moreover, con

    Evaluation of Asymptomatic Malaria Status in Eastern of Afghan-istan Using High Resolution Melting Analysis

    Sayed Hussain MOSAWI, Abdolhossein DALIMI, SAFI Najibullah, Fatemeh GHAFFARIFAR, Javid SADRAEI
    Journal PapersIranian Journal of Parasitology , Volume 15 , Issue 2, 2020 June 7, {Pages 177-186 }

    Abstract

    Background: Malaria is threatening more than half of Afghanistan population. Asymptomatic malaria is notable problem against malaria controlling strategies. In this study we evaluated the asymptomatic malaria status in Nangarhar Province, Afghanistan in 2017. Methods: Overall, 296 finger blood samples were taken on DNA Banking Cards and microscopic slides from asymptomatic individuals in Jalalabad city. We used a novel post real time PCR high resolution melting analysis beside microscopy and semi-nested multiplex PCR to evaluate status of asymptomatic malaria in this city. Results: The prevalence of asymptomatic malaria in Jalalabad city was determined 1.7%(5/296), 7.43%(22/296) and 7.78%(26/296) by microscopy, Seminested multiplex PCR and

    In-vitro and in-vivo comparative effects of the spring and autumn-harvested Artemisia aucheri Bioss extracts on Leishmania major

    Amir Karimipoursaryazdi, Fatemeh Ghaffarifar, Abdolhossein Dalimi, Mohammad Saaid Dayer
    Journal PapersJournal of Ethnopharmacology , 2020 April 25, {Pages 112910 }

    Abstract

    Ethnopharmacological relevanceArtemisia aucheri Bioss contains flavonoid, coumarin and santonin with antioxidant, antimicrobial and antileishmanial effects. The current study was aimed to comparatively evaluate the effects of spring and autumn extracts of A. aucheri Bioss on Leishmania major both in-vitro and in-vivo conditions.MethodsHPLC analysis was used to evaluate the percentages of compounds in spring and autumn extracts of A. aucheri. For in-vitro assay, the effect of different concentrations of spring and autumn extracts of A. aucheri was tested on L. major promastigotes and amastigotes. MTT and flow cytometry methods were used to evaluate the cytotoxicity and probable apoptosis of A. aucheri extracts on L. major promastigotes. On t

    Evaluation of the effects of Rumex obtusifolius seed and leaf extracts against Acanthamoeba: An in vitro study.

    Tooran Nayeri, Farahnaz Bineshian, Fariba Khoshzaban, Abdolhossein Dalimi Asl, Fatemeh Ghaffarifar
    Journal PapersInfectious disorders drug targets , 2020 April 22, {Pages }

    Abstract

    BACKGROUND: Acanthamoebiasis treatment is a major and challenging problem due to the presence of resistant cyst form. Many herbal extracts and their derivatives have been used against trophozoites and cysts of Acanthamoeba, but no effective therapeutic agent has yet been discovered. Therefore, the present study aimed to evaluate the effect of Rumex obtusifolius (R. obtusifolius) extracts against a clinical strain of Acanthamoeba genotype T4 in vitro. METHODS: In this experimental study, after genotyping the clinical isolate, the hydroalcohlic extracts of R. obtusifolius seeds and leaves were prepared. Different concentrations (1.25, 2.5, 5 and 10 mg/ml) of extracts were tested in triplicate (24, 48 and 72h) on trophozoites and cysts of Acan

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    دروس نیمسال جاری

    • دكتري
      ايمونو پارازيتولوژي كاربردي ( واحد)
      دانشکده علوم پزشکی، گروه انگل شناسي
    • دكتري
      ايمونو پارازيتولوژي كاربردي ( واحد)
    • كارشناسي ارشد
      كرم شناسي (2) ( واحد)
    • كارشناسي ارشد
      كرم شناسي (2) ( واحد)

    دروس نیمسال قبل

    • كارشناسي ارشد
      كرم شناسي (1) ( واحد)
      دانشکده علوم پزشکی، گروه انگل شناسي
    • كارشناسي ارشد
      كرم شناسي (1) ( واحد)
    • كارشناسي ارشد
      كارورزي ( واحد)
    • كارشناسي ارشد
      اصول كار با تجهيزات و مواد آزمايشگاهي و ايمني كار ( واحد)
    • كارشناسي ارشد
      كليات ليشمانيوز ( واحد)
    • كارشناسي ارشد
      كليات ليشمانيوز ( واحد)
    • كارشناسي ارشد
      ليشمانيوز پيشرفته ( واحد)
    • كارشناسي ارشد
      ليشمانيوز پيشرفته ( واحد)
    • 1399
      كريمي پور سريزدي, امير
      طراحي DNA كد كننده قطعات مولتي اپي توپي ژن هاي ROP21 و ROP29 به عنوان كانديد واكسن و ارزيابي ايمني زائي آن بر عليه توكسوپلاسموز حاد و مزمن در موش BALB/c
    • مدیر گروه انگل شناسی و حشره شناسی پزشکی دانشکده
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