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  • دکتری (1384)

    انگل شناسی پزشکی

    جامیا / مرکز ملی تحقیقات بیماریهای واگیر دار، دهلی، هند

  • کارشناسی‌ارشد (1368)

    انگل شناسی

    دانشگاه تربیت مدرس، تهران، ایران

  • کارشناسی (1364)

    بهداشت عمومی

    دانشگاه شهید بهشتی، تهران، ایران

  • مطالعه مولکولی انگل های فرصت طلب و تاکسونومی آنها
  • پایش، غربالگری و اپیدمیولوژی انگل های فرصت طلب و درمان با گیاهان دارویی
  • آزمایشگاه انگل‌های فرصت طلب

نام و نام خانوادگي: جاويد صدرايی تاريخ و محل متولد : 1336 ميانه وضعيت تاهل : متاهل داراي دو فرزند تابعيت و مذهب: ايراني و مسلمان وضعيت استخدامي: رسمي- قطعي رتبه علمي: دانشيار محل اشتغال: گروه انگل شناسي دانشكده علوم پزشكي دانشگاه تربيت مدرس تلفن محل كار: 82883841 آدرس الکترونيکي sadraeij@modares.ac.ir sadraeij@yahoo.com مدارك تحصيلي ليسانس بهداشت عمومی از دانشگاه شهيد بهشتی 1364 فوق ليسانس انگل شناسی پزشکی از دانشگاه تربيت مدرس 1368 دکتری انگل شناسی پزشکی از دانشگاه هند 1384 JAMIANICD

Phlebotomus neglectus (Diptera: Psychodidae): New Insights on Its Presence in Iran Based on Three Independent Genetic Loci

M Badakhshan, V Moin-Vaziri, J Sadraei
Journal Papers , , {Pages }

Abstract

A Novel Chimeric Antigen as a Vaccine Candidate against Leishmania major: In silico Analysis

NB Ahmadpour, A Dalimi, M Pirestani, J Sadraei
Journal Papers , , {Pages }

Abstract

In vitro toxicity evaluation of short cationic antimicrobial peptide (CM11) on Blastocystis sp.

Majid Pirestani, Javid Sadraei
Journal PapersActa Tropica , 2020 February 1, {Pages 105384 }

Abstract

Blastocystis infection accounts for one of the causes of gastrointestinal problems with the prevalence rate of 3-100% worldwide. There is a wide range of drugs examined for the treatment of infected patients, among them metronidazole (MTZ) has been introduced as one of the efficient drugs. Besides to the suitable clinical effects, the administration of MTZ has some reported side-effects which emphasize on the identification of putative alternates. To this end, we aimed to evaluate the cytotoxicity effect of a newly-introduced synthetic antimicrobial peptide (AMP) named CM11 on in vitro cultured Blastocystis. Our results exhibited that CM11 treatment affected the viability of parasites in two cultural conditions including culturing alone and

In vitro toxicity evaluation of short cationic antimicrobial peptide (CM11) on Blastocystis sp

Elham Kordestani Shargh, Majid Pirestani, Javid Sadraei
Journal Papers , 2020 January , {Pages }

Abstract

Blastocystis infection accounts for one of the causes of gastrointestinal problems with the prevalence rate of 3–100% worldwide. There is a wide range of drugs examined for the treatment of infected patients, among them metronidazole (MTZ) has been introduced as one of the efficient drugs. Besides to the suitable clinical effects, the administration of MTZ has some reported side-effects which emphasize on the identification of putative alternates. To this end, we aimed to evaluate the cytotoxicity effect of a newly-introduced synthetic antimicrobial peptide (AMP) named CM11 on in vitro cultured Blastocystis. Our results exhibited that CM11 treatment affected the viability of parasites in two cultural conditions including culturing alone a

An unlabeled probe based real time PCR and modified semi nested PCR as molecular tools for analysis of chloroquine resistant in Plasmodium vivax isolates from Afghanistan

A Dalimi, SH Mosawi, N Safi, R Fotouhi-Ardakani, F Ghaffarifar, J Sadraei
Journal Papers , 2020 February 25, {Pages }

Abstract

Background: Chloroquine (CQ) resistance Plasmodium vivax isolates have been reported from many endemic regions in the world. P. vivax has been reported to be about 95% of the whole malaria in Afghanistan and CQ is prescribing in the first-line treatment of vivax malaria. The pvmdr-1 and pvcrt-o (K10 insertion) genes are the possible markers of CQ-resistance P. vivax isolates. There have been no studies done on the prevalence of molecular markers of CQ-resistance P. vivax in Afghanistan. In the present work, we aimed to evaluate the prevalence of mutations in the pvmdr-1 and K10 insertion in the pvcrt-o genes of P. vivax. Methods: P. vivax isolates were collected from Laghman, Baghlan and Khost provinces. For investigation of polymorphisms o

An unlabeled probe based real time PCR and modified semi nested PCR as molecular tools for analysis of chloroquine resistant in Plasmodium vivax isolates from Afghanistan

Sayed Hussain Mosawi, Abdolhossein Dalimi, Najibullah Safi, Reza Fotouhi-Ardakani, Fatemeh Ghaffarifar, Javid Sadraei
Journal Papers , 2020 June 3, {Pages }

Abstract

Background: Plasmodium vivax isolate resistance to Chloroquine (CQ) has been reported from many endemic regions in the world. P. vivax is responsible for 95% of malaria cases in Afghanistan and CQ is the rst-line treatment given for treatment of vivax malaria. The pvmdr-1 and pvcrt-o (K10 insertion) genes are the possible markers for CQ-resistance P. vivax isolates. There have been no studies done on the presence or absence of molecular markers for CQ-resistance P. vivax in Afghanistan. In the present work, we aimed to evaluate the frequency of mutations in the pvmdr-1 and K10 insertion in the pvcrt-o genes of P. vivax.Methods: P. vivax isolates were collected from Laghman, Baghlan and Khost provinces. For investigation of polymorphisms of

Evaluation of Asymptomatic Malaria Status in Eastern of Afghan-istan Using High Resolution Melting Analysis

Sayed Hussain MOSAWI, Abdolhossein DALIMI, SAFI Najibullah, Fatemeh GHAFFARIFAR, Javid SADRAEI
Journal PapersIranian Journal of Parasitology , Volume 15 , Issue 2, 2020 June 7, {Pages 177-186 }

Abstract

Background: Malaria is threatening more than half of Afghanistan population. Asymptomatic malaria is notable problem against malaria controlling strategies. In this study we evaluated the asymptomatic malaria status in Nangarhar Province, Afghanistan in 2017. Methods: Overall, 296 finger blood samples were taken on DNA Banking Cards and microscopic slides from asymptomatic individuals in Jalalabad city. We used a novel post real time PCR high resolution melting analysis beside microscopy and semi-nested multiplex PCR to evaluate status of asymptomatic malaria in this city. Results: The prevalence of asymptomatic malaria in Jalalabad city was determined 1.7%(5/296), 7.43%(22/296) and 7.78%(26/296) by microscopy, Seminested multiplex PCR and

Toxoplasma gondii: Preventive and therapeutic effects of morphine and evaluation of treatment parameters of tachyzoites and infected macrophages in vitro and in a murine model

Leila Zaki, Fatemeh Ghaffarifar, Zohreh Sharifi, John Horton, Javid Sadraei
Journal PapersEXCLI journal , Volume 19 , 2020 January , {Pages 514 }

Abstract

Common medicines for the treatment of toxoplasmosis have limited efficacy and unwanted side effects. Opiates can effect both innate and cell-mediated immunity and stimulate the immune responses in different parasitic infections. In this work, preventive and therapeutic effects of morphine were evaluated on the tachyzoites of Toxoplasma gondii and infected macrophages in vitro and in a murine model. Different concentrations of morphine (0.1 and 0.01 μg/ml) were evaluated on mortality rate of T. gondii by direct counting after 3 and 24 hours. The cytotoxic and apoptotic effects of these drugs were measured by the MTT assays and flow cytometry analysis, respectively. The same procedures were assessed in T. gondii-infected macrophages. The par

Effect of Imiquimod on Tachyzoites of Toxoplasma gondii and Infected Macrophages in vitro and in BALB/c Mice

Leila Zaki, Fatemeh Ghaffarifar, Zohreh Sharifi, John Horton, Javid Sadraei
Journal PapersFrontiers in Cellular and Infection Microbiology , Volume 10 , 2020 January , {Pages }

Abstract

Treatment for toxoplasmosis is not completely successful because of their unwanted side effects, and new treatments are needed. Imiquimod has ability to moderate immune response and used to treat a wide variety of infections and tumors. The aim of the present study was to evaluate the effect of imiquimod on the tachyzoites of T. gondii and infected macrophages in vitro and in BALB/c mice. The viability of T. gondii was assessed in the presence of various concentrations of imiquimod by direct counting after 6 and 24 h. The MTT assay was used to identify the viability of uninfected macrophages. The apoptotic effects were determined with flow cytometry on the tachyzoites and infected macrophages. For evaluation of parasite load in pre-treatmen

Evaluation the Effect of Imiquimod on Tachyzoites of Toxoplasma gondii and Infected Macrophages In Vitro and In Vivo

Fatemeh Ghaffarifar, Leila Zaki, Zohreh Sharifi, John Horton, Javid Sadraei
Journal PapersFrontiers in Cellular and Infection Microbiology , Volume 10 , 2020 January , {Pages 387 }

Abstract

The commonly prescribed drugs for toxoplasmosis have not yet been completely successful because of their unwanted side effects. Imiquimod has ability to moderate immune response and used to treat a wide variety of infections and tumors. The aim of the present study was to evaluate the effect of imiquimod on the tachyzoites of Toxoplasma gondii and infected macrophages in vitro and in vivo. The viability of T. gondii was assessed in the presence of various concentrations of imiquimod by direct counting after 3 and 24 hours. The MTT assay was used to identify the viability of uninfected macrophages. The apoptotic effects of this drug was determined with flow cytometry assay on the tachyzoites and infected macrophages, respectively. For evalua

Effect of Imiquimod on Tachyzoites ofToxoplasma gondiiand Infected Macrophagesin vitroand in BALB/c Mice

Leila Zaki, Fatemeh Ghaffarifar, Zohreh Sharifi, John Horton, Javid Sadraei
Journal PapersFRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY , Volume 10 , 2020 July 31, {Pages }

Abstract

Treatment for toxoplasmosis is not completely successful because of their unwanted side effects, and new treatments are needed. Imiquimod has ability to moderate immune response and used to treat a wide variety of infections and tumors. The aim of the present study was to evaluate the effect of imiquimod on the tachyzoites ofT. gondiiand infected macrophagesin vitroand in BALB/c mice. The viability ofT. gondiiwas assessed in the presence of various concentrations of imiquimod by direct counting after 6 and 24 h. The MTT assay was used to identify the viability of uninfected macrophages. The apoptotic effects were determined with flow cytometry on the tachyzoites and infected macrophages. For evaluation of parasite load in pre-treatment or

Blastocystis sp. Subtype 9: as the First Reported Subtype in Patients with Schizophrenia in Iran

Shaghayegh Sheikh, Ali Asghari, Javid Sadraei, Majid Pirestani, Maryam Zare
Journal PapersSN Comprehensive Clinical Medicine , Volume 2 , 2020 May , {Pages 633-639 }

Abstract

Blastocystis sp. is more prevalent in mentally-retarded individuals, immunocompromised patients, and organ recipients. There are no inclusive studies in Iran evaluating the prevalence and molecular identification of Blastocystis sp. infection in schizophrenic patients. This study aimed to determine prevalence and subtype distribution of Blastocystis sp. in schizophrenic patients in Tehran province, Iran. This cross-sectional study was performed on 58 stool samples of schizophrenic male patients in Tehran province, Iran, using the sequence-tagged site (STS) and ST-specific primers. Overall, 58 stool samples were collected from hospitalized male patients with schizophrenia in Tehran province. After conventional PCR, positive sa

An unlabelled probe-based real time PCR and modified semi-nested PCR as molecular tools for analysis of chloroquine resistant Plasmodium vivax isolates from Afghanistan

SH Mosawi, A Dalimi, N Safi, R Fotouhi-Ardakani, F Ghaffarifar, J Sadraei
Journal Papers , , {Pages }

Abstract

Evaluation of asymptomatic malaria status in eastern of Afghanistan using high resolution melting analysis

SH Mosawi, A Dalimi, S Najibullah, F Ghaffarifar, J Sadraei
Journal Papers , , {Pages }

Abstract

In vitro toxicity evaluation of short cationic antimicrobial peptide (CM11) on Blastocystis sp

EK Shargh, M Pirestani, J Sadraei
Journal Papers , , {Pages }

Abstract

First molecular identification and subtype distribution of Blastocystis sp. isolated from hooded crows (Corvus cornix) and pigeons (Columba livia) in Tehran Province, Iran

Ali Asghari, Javid Sadraei, Majid Pirestani, Iraj Mohammadpour
Journal PapersComparative immunology, microbiology and infectious diseases , Volume 62 , 2019 February 1, {Pages 25-30 }

Abstract

Blastocystis is a common intestinal parasite among humans and animals such as non-human primates, pigs, cattle, birds, amphibians, and less frequently, rats, reptiles and insects. Since Blastocystis is a widely transmissible parasite between humans and mammals or birds, it is prominent to determine whether newly secluded non-human isolates are zoonotic. There are no comprehensive studies in Iran assessing the prevalence and molecular identification of Blastocystis infection in birds, especially in pigeons and crows. So, the aim of this study was to identify Blastocystis subtypes (STs) in crows and pigeons in Tehran province, Iran, using Nested PCR-RFLP and sequencing. Overall, 300 Blastocystis isolates from birds (156 pigeons and 144 crows)

First isolation of Toxoplasma gondii from rodents and stray cats in northeast of Iran

Ehsan Shariat Bahadori, Javid Sadraei, Abdolhosein Dalimi, Somayyeh Namroodi, Majid Pirestani
Journal PapersINDIAN JOURNAL OF ANIMAL SCIENCES , Volume 89 , Issue 5, 2019 January 1, {Pages 485-490 }

Abstract

Toxoplasma gondii is a common coccidian parasite with stray cat family as definitive host, and a wide range of warm blooded animals and human as intermediate hosts. The objective of this study was to isolate T. gondii from rodents and stray cats in northeast of Iran. Genomic DNA was extracted from 30-50 mg of the rodents and stray cats' tissue using phenol-chloroform extraction method. Using PCR-RFLP, the genes (SAG1, SAG3, GRA6, 18SrRNA) and sequencing method, the isolation of T. gondii from these hosts was determined. ELISA method was also used on serum samples to evaluate the IgG antibodies against T. gondii among the hosts. Out of 286 rodents and 210 stray cats tested, the DNA of T. gondii was detected in 68 and 38 samples, respectivel

Effect of Cecropin–melittin Chimeric Peptide (CM11) on Trophozoite of Giardia lamblia In Vitro

Somayeh Karimi Tanha, Majid Pirestani, Javid Sadraei
Journal PapersJournal of Mazandaran University of Medical Sciences , Volume 29 , Issue 177, 2019 October 10, {Pages 42-55 }

Abstract

Background and purpose: Antimicrobial peptides (AMP) are one of the most diverse antimicrobial compounds that have received much attention due to the development of drug resistance of pathogens to conventional antibiotics. But, few studies have evaluated anti-parasitic properties of AMP. The present study was conducted to compare the effect of a cecropin–melittin chimeric peptide (CM11) and metronidazole on Giardia lamblia trophozoite.Materials and methods: In this study, using MTT assay, the cytotoxicity of CM-11 peptide (0.5-32 μg/ml) and metronidazole (0.25-25 μg/ml) was investigated on Caco-2 cells and IC50 was calculated. Flow cytometry was used to determine the rate of induction of apoptosis by CM11 and metronidazole. The toxicity

In-vitro and In-vivo Antileishmanial Activity of a Compound Derived of Platinum, Oxaliplatin, against Leishmania Major

Ezatollah Ghasemi, Fatemeh Ghaffarifar, Abdolhossein Dalimi, Javid Sadraei
Journal PapersIranian Journal of Pharmaceutical Research , 2019 September 3, {Pages }

Abstract

This study aimed to evaluate the antileishmanial efficacy of oxaliplatin against Leishmania major both in-vitro and in-vivo. The IC 50, CC 50, and SI of oxaliplatin against promastigotes, murine macrophages, Raw 264.7 cells, and intramacrophage amastigotes of L. major were investigated in-vitro. The effects of this drug on intracellular amastigotes were also assayed, and the percentage of infectivity and IIR were calculated. Flow cytometry was performed to assay apoptosis, using 50 and 100 ?g/mL of oxaliplatin in the promastigotes and macrophages. In-vivo, the BALB/c mice were classified into three groups, receiving oxaliplatin, glucantime, and phosphate-buffered saline for one month, respectively. The lesion size, IFN-γ, and IL-4 levels,

New prevalence surveillance of Toxoplasma gondii among rodents and stray cats by ELISA avidity and nested PCR methods, Northeast of Iran

Ehsan Shariat Bahadori, Javid Sadraei, Abdolhosein Dalimi
Journal PapersJournal of Parasitic Diseases , Volume 43 , Issue 3, 2019 September 1, {Pages 360-367 }

Abstract

Rodents and stray cats are the sources of many parasitic infections including T. gondii, for other animals and human. Toxoplasmosis has a wide range of laboratory factors in its intermediate and definite hosts. Regarding the importance of rodents and stray cats as the hosts that spread the Toxoplasma gondii, it is necessary to obtain comprehensive information about these animals in the life cycle of T. gondii. The objective was to investigate the new prevalence of toxoplasmosis among target animals in Iran, using GRA6 gene in combinacion with ELISA avidity. In this study, 286 rodents and 210 stray cats were collected and their heart tissues extracted to obtain DNA, blood samples and IgG Ab of T. gondii parasite. We detected the positive tis

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دروس نیمسال جاری

  • دكتري
    فارماكولوژي داروهاي ضد انگلي ( واحد)
    دانشکده علوم پزشکی، گروه انگل شناسي
  • كارشناسي ارشد
    اصول اپيدميولوژي ( واحد)
  • كارشناسي ارشد
    كرم شناسي (2) ( واحد)
  • كارشناسي ارشد
    كرم شناسي (2) ( واحد)

دروس نیمسال قبل

  • كارشناسي ارشد
    حيوانات آزمايشگاهي و كاربرد آن در انگل شناسي ( واحد)
    دانشکده علوم پزشکی، گروه انگل شناسي
  • كارشناسي ارشد
    حيوانات آزمايشگاهي و كاربرد آن در انگل شناسي ( واحد)
  • كارشناسي ارشد
    كرم شناسي (1) ( واحد)
  • كارشناسي ارشد
    كرم شناسي (1) ( واحد)
  • كارشناسي ارشد
    روش تحقيق در علوم بهداشتي ( واحد)
  • كارشناسي ارشد
    روش تحقيق در علوم بهداشتي ( واحد)
  • كارشناسي ارشد
    كارورزي ( واحد)
  • كارشناسي ارشد
    اصول كار با تجهيزات و مواد آزمايشگاهي و ايمني كار ( واحد)
  • 1400
    غياثي پور, الهه
    بررسي اثردارويي 2 نيتروايميدازول برروي انگل توكسوپلاسما گونده اي درشرايط آزمايشگاهي(invitro)وموشinvivo Balb/c
  • 1400
    يزدي زاده خليلي, جواد
  • 1395
    گرگي پور, محمد
    بررسي رشد ساب تايپ هاي 1و 3 بلاستوسيستيس در حضور لاكتوباسيلوس اسيدوفيلوس ، لاكتوباسيلوس روتري و بيفيديوباكتريوم بيفيدوم از طريق سنجش TLR2 و TLR4 در محيط كشت سلولي Caco2
  • 1397
    شهريور كلوانق, فيروز
  • مدیر گروه انگل شناسی وحشر شناسی
  • مدیر گروه انگل شناسی وحشر شناسی
  • عضو شورای نظارت دانشکده علوم پزشکی
  • مدیر گروه آموزشی انگل شناسی پزشکی
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