Campylobacter jejuni (C. jejuni) is a leading cause of acute gastroenteritis in human worldwide. The aim of study was to assess the distribution of sialylated lipooligosaccharide (LOS) classes and capsular genotypes in C. jejuni isolated from Iranian children with gastroenteritis. Furthermore, the level of dnaK gene expression in C. jejuni strains with selected capsular genotypes and LOS classes was intended. Moreover, a comprehensive study of C. jejuni MLST-genotypes and inclusive comparison with peer sequences worldwide was intended. Twenty clinical C. jejuni strains were isolated from fecal specimens of 280 children aged 0–5 years, suspected of bacterial gastroenteritis, which admitted to 3 children hospitals from May to October, 201
Background: Cholera could cause severe watery diarrhea and even death, but in patients diagnosed with severe dehydration, treatment with an appropriate antimicrobial agent could decrease the volume of diarrhea and shorten the duration of V. cholerae shedding. Unfortunately, due to the high antibiotic’s consumption, antibiotic resistance in V. cholerae strains is progressively increasing worldwide. The present systematic review and meta-analysis study aimed to investigate the incidence of antibiotic resistance in V. cholerae strains worldwide.Methods: The incidence of antibiotic resistance in V. cholerae strains was assessed by conducting a systematic review and searching international databases including PubMed and Google Scholar for arti
Prevalence of intestinal parasitic infections and Campylobacter spp. among children with gastrointestinal disorders in Tehran, Iran
Mohammad Barati, Ali Taghipour, Bita Bakhshi, Saeed Shams, Majid Pirestani
Journal PapersParasite Epidemiology and Control , 2021 February 14, {Pages e00207 }
Abstract
Co-infection of intestinal parasitic infections (IPIs) and Campylobacter spp. are public health problem in both developing and developed countries. This study was conducted to determine prevalence of IPIs and Campylobacter spp. among children with gastrointestinal disorders in Tehran. In this descriptive cross-sectional survey, 283 fresh stool samples were collected from all individuals and examined by standard parasitological methods including direct slide smear, formalin-ether concentration, trichrome staining, modified Ziehl-Neelsen staining, and chromotrope 2R staining techniques were used for detection of intestinal protozoa and helminths. Furthermore, culture and multiplex-PCR were also used to identify the species of Campylobacter. D
Fabrication, tuning and performance analysis of polyacrylonitrile (PAN)-derived microfiltration membranes for bacteria removal from drinking water
Seyed Saeid Hosseini, Hadi Khodadadi, Bita Bakhshi
Journal PapersKorean Journal of Chemical Engineering , Volume 38 , Issue 1, 2021 January , {Pages 32-45 }
Abstract
Removal of bacterial contaminations from water using advanced technologies is one of the essential steps in improving human health. The present study’s aim was to develop high performance microfiltration membranes from polyacrylonitrile (PAN) for bacteria removal from drinking water. The characteristics and performance of membranes were tuned through exploring the variation of prominent fabrication and operating parameters. The findings reveal that increasing PAN concentration in dope and addition of citric acid were successful in tailoring membrane microstructure. Bacteria rejection in modified membranes improved by exhibiting high log removal values (LRV) ranging from 3.92 (99.87%) to 5.57 (99.99%) while permeate fluxes w
Some Staphylococcus aureus strains produce Panton-Valentine leukocidin (PVL), a bi-component pore-forming toxin, which causes leukocyte lysis and tissue necrosis. Currently, there is very limited information on the molecular epidemiology of PVL-encoding S. aureus strains in Iran. This study aimed to determine the molecular epidemiology and genetic background of PVL-positive S. aureus clinical strains isolated from Iranian patients. A total of 28 PVL-positive S. aureus strains were detected from 600 S. aureus isolates between February 2015 and March 2018 from different hospitals in Tehran, Iran. Antimicrobial susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Molecular genot
Bioactive glasses (BGs) have attracted added attention in the structure of the scaffolds for bone repair applications. Different metal ions could be doped in BGs to induce specific biological responses. Among these ions, strontium (Sr) is considered as an effective and safe doping element with promising effects on bone formation and regeneration. In this experiment, we evaluated the antibacterial activities of the gelatin-BG (Gel-BG) and Gel-BG/Sr scaffolds in vitro. The osteogenic properties of the prepared scaffolds were also assessed in rabbit calvarial bone defects for 12 weeks. Both scaffolds showed in vivo bone formation during 12 weeks with the newly formed bone area in Gel-BG/Sr scaffold was higher than that in Gel-BG scaffolds afte
Vibrio cholerae toxin co‐regulated pilus provokes inflammatory responses in co‐culture model of Caco‐2 and Peripheral Blood Mononuclear Cells (PBMC) leading?…
Maryam Ghasemi, Bita Bakhshi, Reza Khashei, Sara Soudi, Mina Boustanshenas
Journal PapersMicrobiology and Immunology , 2021 April 29, {Pages }
Abstract
The aim of this study was to assess the modulatory effect of TcpA in the expression of CEACAM1 adhesin molecule and IL‐1, IL‐8 and TNFα pro‐inflammatiory cytokines in the co‐culture model of Caco‐2/PBMC that can mimic the intestinal milieu. The tcpA gene from V.cholerae ATCC14035 was cloned in pET‐28a and transformed into E.coli Bl‐21. The recombinant TcpA‐His6 protein was expressed and purified using the Ni‐column chromatography. The sequencing of transformed plasmid and Western blotting of purified protein confirmed the identity of rTcp. Cytotoxicity of different concentrations of recombinant protein for human colon carcinoma cell line (Caco‐2 cell) was assessed by MTT assay and showed viability of 92%, 82% and 70 %,
Investigating the effect of an identified mutation within a critical site of PAS domain of WalK protein in a vancomycin-intermediate resistant Staphylococcus aureus by …
Significant contribution of the CmeABC Efflux pump in high-level resistance to ciprofloxacin and tetracycline in Campylobacter jejuni and Campylobacter coli clinical isolates
Vibrio cholerae toxin coregulated pilus provokes inflammatory responses in Coculture model of Caco‐2 and peripheral blood mononuclear cells (PBMC) leading to …
M Ghasemi, B Bakhshi, R Khashei, S Soudi, M Boustanshenas
The effect of subinhibitory concentration of chlorhexidine on the evolution of vancomycin-intermediate Staphylococcus aureus and the induction of mutations in walKR and vraTSR …
Isolation and Kinetic Modeling of New Culture from Compost with High Capability of Degrading n-Hexadecane, Focused on Ochrobactrum Oryzae and Paenibacillus Lautus
Mohammad Reza Samaei, Mahrokh Jalili, Fariba Abbasi, Sayed Bagher Mortazavi, Ahmad Jonidi Jafari, Bita Bakhshi
Journal PapersSoil and Sediment Contamination: An International Journal , 2020 February 2, {Pages 13-Jan }
Abstract
Nowadays, petroleum pollution is one of the most important environmental challenges in Iran. Some bacteria were isolated for hexadecane degrading from soil, sediment, and sludge; however, there is no report on its isolation from compost. This study was aimed to isolation, molecular identification of novel bacteria with high capability of hexadecane-degrading from compost using enrichments media. The isolated bacteria were identified by PCR with 16S rDNA method and were studied their ability for removing hexadecane in liquid and solid medium. According to results, the isolated bacteria were identified as O.oryzae and P.lautus. In liquid medium, hexadecane concentration decreased from 3000?1.4 to 366.96?0.9 mg/l (87.77?0.2%) by O.oryzae, whil
بررسي ارتباط ژنتيكي و فيلوژنيكي ايزوله هاي ويبريو كلرا جدا شده از طغيان هاي سال 1401 ايران با پاندمي هفتم وبا با استفاده از تكنيك MLST
1396
شهبازي, طيبه
تعيين ميكروبيوتاي گوارشي غالب در بيماران مبتلا به سرطان كلوركتال (CRC) به روش 16S rRNA metagenomic و بررسي اثر سوپرناتانت محتويات روده اين افراد بر افزايش بيان ژنهاي مرتبط با CRC در رده سلولي Caco2
1398
حيدري, ليلا
بررسي اثر آنتي ميكروبيال نانوپارتيكل كيتوزان حامل سوپ سلولي حاصل ازسلولهاي بنيادي مزانشيمال مشتق ازمغز استخوان بر اسينتوباكتر بوماني مقاوم به كارباپنم (CRAB) در مدل موشي
1398
درخشان سفيدي, مژگان
بررسي نقش كوكتل پروبيوتيكي متشكل از Lactobacillus acidophilus و Bifidobacterium bifidum و نانوسامانه Nisin/سلنيوم/كيتوزان در بهبود اثر خوراكي داروي 5FU بر سرطان كولوركتال در مدل موشيBALB/c
1400
گودرزي, فروغ
بررسي نقش اسپور باسيلوس كوآگولانس در افزايش اثر نانوذره مزوپروس كونژوگه شده با داروي فلورواوراسيل در درمان سرطان كولوركتال در مدل موشي BALB/c